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Selection of family 1 PspA molecules capable of inducing broad-ranging cross-reactivity by complement deposition and opsonophagocytosis by murine peritoneal cells.
- Source :
-
Vaccine [Vaccine] 2011 Feb 11; Vol. 29 (8), pp. 1634-42. Date of Electronic Publication: 2011 Jan 04. - Publication Year :
- 2011
-
Abstract
- PspA is one of the most well studied pneumococcal proteins and a promising candidate for a future protein-based anti-pneumococcal vaccine. Nevertheless, its structural and serological variability suggests the inclusion of more than one PspA molecule in order to broaden protection. Since different PspAs exhibit variable levels of cross-reactivity, the selection of the protein combination with the highest coverage potential is an essential step for PspA-based vaccine development. This work investigated the level of cross-reactivity within family 1 PspAs, and established a complement based antibody mediated opsonophagocytic assay for measuring the level of cross-protection. Among a panel of ten family 1 PspA molecules, two of them, one belonging to clade 1 and another from clade 2, induced antibodies capable of enhancing complement deposition and mediating the phagocytic killing by mouse peritoneal macrophages of all pneumococci bearing PspA family 1 strains tested, regardless of their serotype. Therefore, we suggest the inclusion of either one in a PspA-based vaccine, as a representative of family 1. Furthermore, our results suggest that opsonophagocytosis by mouse peritoneal cells can be an efficient means of evaluating the induction of protective immune responses in mice across a large number of strains.<br /> (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibodies, Bacterial blood
Antibodies, Bacterial immunology
Antigens, Bacterial immunology
Cross Reactions immunology
Female
Mice
Mice, Inbred BALB C
Pneumococcal Infections immunology
Pneumococcal Infections prevention & control
Pneumococcal Vaccines immunology
Streptococcus pneumoniae immunology
Bacterial Proteins immunology
Complement System Proteins immunology
Cross Protection
Macrophages, Peritoneal immunology
Phagocytosis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 29
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 21211592
- Full Text :
- https://doi.org/10.1016/j.vaccine.2010.12.074