Determination of sphingomyelinase-D activity of Loxosceles venoms in sphingomyelin/cholesterol liposomes containing horseradish peroxidase.

Authors :: Gomes MT
Guimarães G
Frézard F
Kalapothakis E
Minozzo JC
Chaim OM
Veiga SS
Oliveira SC
Chávez-Olórtegui C
Source :: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2011 Mar 15; Vol. 57 (4), pp. 574-9. Date of Electronic Publication: 2011 Jan 12.
Publication Year :: 2011
Abstract: Based on degradation of sphingomyelin/cholesterol liposomes containing entrapped horseradish peroxidase, we evaluated the Sphingomyelinase-D (SMase-D) activity of scorpion, spider and snake venoms by monitoring spectrophotometrically the product of oxidation of HRP released. The results indicate that Loxosceles crude venoms (Loxosceles intermedia, Loxosceles laeta, Loxosceles gaucho and Loxosceles similis) displayed SMase-D activity in a concentration-dependent manner. Furthermore, this activity was blocked by the anti-loxoscelic antivenom. However, Tityus serrulatus scorpion venom, Phoneutria nigriventer spider venom and Bothrops jararaca, Crotalus durissus, Lachesis muta and Micrurus frontalis snake venoms did not show measurable SMase-D activity.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)

Subjects :: Animals
Antivenins pharmacology
Cholesterol chemistry
Cholesterol metabolism
Horseradish Peroxidase metabolism
Liposomes chemistry
Oxidation-Reduction
Recombinant Proteins
Species Specificity
Sphingomyelins chemistry
Sphingomyelins metabolism
Spider Venoms toxicity
Liposomes metabolism
Phosphoric Diester Hydrolases metabolism
Spider Venoms enzymology
Spiders classification

Language :: English
ISSN :: 1879-3150
Volume :: 57
Issue :: 4
Database :: MEDLINE
Journal :: Toxicon : official journal of the International Society on Toxinology
Publication Type :: Academic Journal
Accession number :: 21236288
Full Text :: https://doi.org/10.1016/j.toxicon.2011.01.001

Full Text Access

Tools