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RNAi screening for fat regulatory genes with SRS microscopy.
- Source :
-
Nature methods [Nat Methods] 2011 Feb; Vol. 8 (2), pp. 135-8. Date of Electronic Publication: 2011 Jan 16. - Publication Year :
- 2011
-
Abstract
- Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage.
- Subjects :
- Animals
Caenorhabditis elegans chemistry
Caenorhabditis elegans metabolism
Fats metabolism
Microscopy instrumentation
Spectrum Analysis, Raman instrumentation
Caenorhabditis elegans genetics
Fats analysis
Gene Expression Regulation
Microscopy methods
RNA Interference
Spectrum Analysis, Raman methods
Subjects
Details
- Language :
- English
- ISSN :
- 1548-7105
- Volume :
- 8
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Nature methods
- Publication Type :
- Academic Journal
- Accession number :
- 21240281
- Full Text :
- https://doi.org/10.1038/nmeth.1556