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An ethanol extract of Iris nertschinskia induces p53-dependent apoptosis in the MCF7 human breast cancer cell line.
- Source :
-
International journal of molecular medicine [Int J Mol Med] 2011 Mar; Vol. 27 (3), pp. 401-5. Date of Electronic Publication: 2011 Jan 14. - Publication Year :
- 2011
-
Abstract
- Iris nertschinskia, an ornamental plant, is utilized in traditional East Asian medicine for the treatment of skin diseases. However, the biological activity underlying its therapeutic effects remains to be established. In this study, we investigated the anti-tumor effect of the plant extract on MCF7 human breast cancer cells. An ethanol extract of Iris nertschinskia triggered cell death in a dose-dependent manner. Moreover, treatment with the extract promoted p53 phosphorylation in MCF7 cells. Increased phosphorylation of p53, in turn, led to induction of Bax protein, a key regulator of p53-dependent apoptotic cell death, as well as of caspase-7 cleavage in MCF7 cells. Consistently, cells treated with p53-specific siRNA or the caspase inhibitor, Z-VAD, resisted apoptotic cell death induced by the Iris nertschinskia extract. Our results suggest that p53 sensitizes tumor cells to the ethanol extract of Iris nertschinskia by Bax protein induction and caspase-dependent apoptosis.
- Subjects :
- Breast Neoplasms drug therapy
Breast Neoplasms pathology
Caspase 7 metabolism
Cell Line, Tumor
Drug Screening Assays, Antitumor
Ethanol chemistry
Female
Humans
Phosphorylation drug effects
Plant Extracts chemistry
bcl-2-Associated X Protein metabolism
Apoptosis drug effects
Breast Neoplasms metabolism
Iris Plant chemistry
Plant Extracts pharmacology
Tumor Suppressor Protein p53 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1791-244X
- Volume :
- 27
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 21240456
- Full Text :
- https://doi.org/10.3892/ijmm.2011.599