Development and validation of I-DNA1: a 15-Loci multiplex system for identity testing.

Authors :: Odriozola A
Aznar JM
Celorrio D
Bravo ML
Builes JJ
Val-Bernal JF
de Pancorbo MM
Source :: International journal of legal medicine [Int J Legal Med] 2011 Sep; Vol. 125 (5), pp. 685-94. Date of Electronic Publication: 2011 Jan 22.
Publication Year :: 2011
Abstract: This paper presents a system for the multiplex amplification of 15 loci, known as I-DNA1, which combines mini and midiSTR technology, with amplicon sizes ranging from 49 to 297 bp. I-DNA1 analyses all the STR loci included in the CODIS and the Interpol Standard Set of loci, nine of the ten European core loci and seven of the eight German core loci, making it suitable for use in identifying humans. Moreover, its high sensitivity and the small size of its amplicons mean that I-DNA1 is potentially highly useful for analysing highly degraded and/or very small DNA samples.

Subjects :: DNA Fingerprinting statistics & numerical data
Female
Gene Frequency genetics
Humans
Liver pathology
Male
Myocardium pathology
Postmortem Changes
Reproducibility of Results
Spain
DNA Fingerprinting legislation & jurisprudence
DNA Fingerprinting methods
Forensic Anthropology legislation & jurisprudence
Forensic Anthropology methods
Genetic Loci genetics
Microsatellite Repeats genetics
Multiplex Polymerase Chain Reaction methods
Multiplex Polymerase Chain Reaction statistics & numerical data
Reagent Kits, Diagnostic statistics & numerical data

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