Back to Search
Start Over
[Rapid determination of ethanol in blood by capillary-gas chromatography].
- Source :
-
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition [Sichuan Da Xue Xue Bao Yi Xue Ban] 2010 Nov; Vol. 41 (6), pp. 1055-8. - Publication Year :
- 2010
-
Abstract
- Objective: To develop a method for the rapid determination of ethanol in blood with capillary-GC.<br />Methods: 0.50 mL of whole blood sample was taken and added with 1.00 mL of dimethyl sulfoxide (DMSO), and 2 g of anhydrous sodium sulfate. The supernatant of the sample solution was directly injected into GC for analysis.<br />Results: Ethanol was separated from other substances in the sample. The liner range of ethanol detected by the capillary-GC was 0.0-300.0 mg/100 mL, and the detection limit was 0.2 mg/100 mL. The RSD for standard solution determination was 1.36%. Satisfactory results were obtained for the determination of ethanol in whole blood samples, with recoveries ranging from 90.9% to 107.3% and a RSD of 1.98%. The combined uncertainty was 2.2%.<br />Conclusion: This is a rapid, sensitive and simple method for determination of ethanol in large quantities of samples. The method has shortened the duration of analysis cycle in comparison with the traditional headspace-GC, with a reduction from 20-30 min to less than 10 min.
- Subjects :
- Humans
Sensitivity and Specificity
Chromatography, Gas methods
Ethanol blood
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1672-173X
- Volume :
- 41
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
- Publication Type :
- Academic Journal
- Accession number :
- 21265115