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Fast and sensitive LC-DAD-ESI/MS method for analysis of saikosaponins c, a, and d from the roots of Bupleurum Falcatum (Sandaochaihu).
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2011 Feb 11; Vol. 16 (2), pp. 1533-43. Date of Electronic Publication: 2011 Feb 11. - Publication Year :
- 2011
-
Abstract
- In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 x 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of saikosaponins c, a and d from the roots of Bupleurum falcatum.
- Subjects :
- Chromatography, Liquid instrumentation
Limit of Detection
Medicine, Chinese Traditional
Molecular Structure
Oleanolic Acid analysis
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization instrumentation
Bupleurum chemistry
Chromatography, Liquid methods
Oleanolic Acid analogs & derivatives
Plant Roots chemistry
Saponins analysis
Spectrometry, Mass, Electrospray Ionization methods
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 16
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 21317843
- Full Text :
- https://doi.org/10.3390/molecules16021533