Back to Search
Start Over
Supercritical angle fluorescence immunoassay platform.
- Source :
-
Analytical chemistry [Anal Chem] 2011 Mar 15; Vol. 83 (6), pp. 2345-50. Date of Electronic Publication: 2011 Feb 21. - Publication Year :
- 2011
-
Abstract
- An inexpensive and easy-to-use immunoassay platform for the sensitive detection of analytes is presented. It comprises single-use polymer test tubes and a compact fluorescence reader. The optics for the capture of supercritical angle fluorescence (SAF) has been built into the tubes allowing for the extremely sensitive readout of solid phase immunoassays in real time and without washing steps. One-step sandwich immunoassays with interleukin 2 (IL-2) were carried out with capture antibodies immobilized in the tubes. At a turn around time of 12 min, the limit of detection for IL-2 was 0.27 pM (4.5 pg/mL) and the linear range covered 3 orders of magnitude. The developed technology is also adaptable to well plates and has great potential of replacing the work-intensive and time-consuming enzyme-linked immunosorbant assay (ELISA).
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 83
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 21338064
- Full Text :
- https://doi.org/10.1021/ac1032758