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In situ hybridization of whole-mount mouse embryos with RNA probes: hybridization, washes, and histochemistry.
- Source :
-
CSH protocols [CSH Protoc] 2007 Nov 01; Vol. 2007, pp. pdb.prot4823. Date of Electronic Publication: 2007 Nov 01. - Publication Year :
- 2007
-
Abstract
- INTRODUCTIONThis is the second of two protocols describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate.
Details
- Language :
- English
- Volume :
- 2007
- Database :
- MEDLINE
- Journal :
- CSH protocols
- Publication Type :
- Academic Journal
- Accession number :
- 21356976
- Full Text :
- https://doi.org/10.1101/pdb.prot4823