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Functional modulation of choroid plexus epithelial clusters in vitro for tissue repair applications.
- Source :
-
Cell transplantation [Cell Transplant] 2011; Vol. 20 (11-12), pp. 1659-72. Date of Electronic Publication: 2011 Mar 08. - Publication Year :
- 2011
-
Abstract
- One of the primary obstacles in the restoration or repair of damaged tissues is the temporospatial orchestration of biological and physiological events. Cellular transplantation is an important component of tissue repair as grafted cells can serve as replacement cells or as a source of secreted factors. But few, if any, primary cells can perform more than a single tissue repair function. Epithelial cells, derived from the choroid plexus (CP), are an exception to this rule, as transplanted CP is protective and regenerative in animal models as diverse as CNS degeneration and dermal wound repair. They secrete a myriad of proteins with therapeutic potential as well as matrix and adhesion factors, and contain responsive cytoskeletal components potentially capable of precise manipulation of cellular and extracellular niches. Here we isolated CP from neonatal porcine lateral ventricles and cultured the cells under a variety of conditions to specifically modulate tissue morphology (2D vs. 3D) and protein expression. Using qRT-PCR analysis, transmission electron microscopy, and gene microarray studies we demonstrate a fine level of control over CP epithelial cell clusters opening further opportunities for exploration of the therapeutic potential of this unique tissue source.
- Subjects :
- Animals
Cell Culture Techniques
Cells, Cultured
Central Nervous System physiology
Choroid Plexus metabolism
Collagen chemistry
Dermis physiology
Drug Combinations
Epithelial Cells metabolism
Epithelial Cells transplantation
Gene Expression Regulation
Laminin chemistry
Lateral Ventricles cytology
Models, Animal
Prealbumin genetics
Prealbumin metabolism
Proteoglycans chemistry
RNA, Messenger metabolism
Regeneration
Swine
Vascular Endothelial Growth Factor A genetics
Vascular Endothelial Growth Factor A metabolism
Wound Healing
Choroid Plexus cytology
Epithelial Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1555-3892
- Volume :
- 20
- Issue :
- 11-12
- Database :
- MEDLINE
- Journal :
- Cell transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 21396169
- Full Text :
- https://doi.org/10.3727/096368911X564985