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Alpinumisoflavone induces apoptosis and suppresses extracellular signal-regulated kinases/mitogen activated protein kinase and nuclear factor-κB pathways in lung tumor cells.
- Source :
-
Biological & pharmaceutical bulletin [Biol Pharm Bull] 2011; Vol. 34 (2), pp. 203-8. - Publication Year :
- 2011
-
Abstract
- The extracellular signal-regulated kinases/mitogen activated protein kinase (ERK/MAPK) and nuclear factor-κB (NF-κB) pathways are critical for cell survival and proliferation. Alpinumisoflavone (AIF), isolated from the African medicinal plant Erythrina lysistemon, is a member of the isoflavone group. In this report, we demonstrated that AIF treatment induces cell death of human lung tumor cells. Incubation of lung tumor cells with AIF increased the sub-G1 population and caspase 3/7 activity, suggesting that the cell death is caused by apoptosis. To identify the signaling pathway involved in the tumor cell death, we examined the modulation of transcriptional activity using various reporter constructs and found that AIF significantly deregulated both the ERK/MAPK and NF-κB pathways. Western blot analysis with antibodies to MAP/ERK kinase (MEK) and ERK showed that AIF dephosphorylates both MEK and ERK. Alpinumisoflavone also repressed lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 cells by inhibiting NF-κB-dependent transcription. Therefore, the cell death induced by AIF may be via repressing both the ERK/MAPK and NF-κB pathways.
- Subjects :
- Animals
Antineoplastic Agents, Phytogenic isolation & purification
Antineoplastic Agents, Phytogenic pharmacology
Antineoplastic Agents, Phytogenic therapeutic use
Caspases metabolism
Cell Cycle drug effects
Cell Line, Tumor
Gene Expression drug effects
Humans
Isoflavones isolation & purification
Isoflavones therapeutic use
Lipopolysaccharides
Lung Neoplasms metabolism
Lung Neoplasms pathology
Mice
Nitric Oxide biosynthesis
Phosphorylation
Phytotherapy
Plant Extracts chemistry
Plant Extracts therapeutic use
Signal Transduction drug effects
Apoptosis drug effects
Erythrina chemistry
Extracellular Signal-Regulated MAP Kinases antagonists & inhibitors
Isoflavones pharmacology
Lung Neoplasms drug therapy
NF-kappa B antagonists & inhibitors
Plant Extracts pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1347-5215
- Volume :
- 34
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biological & pharmaceutical bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 21415528
- Full Text :
- https://doi.org/10.1248/bpb.34.203