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Evaluation of a broadly protective Chlamydia-cholera combination vaccine candidate.
- Source :
-
Vaccine [Vaccine] 2011 May 12; Vol. 29 (21), pp. 3802-10. Date of Electronic Publication: 2011 Mar 21. - Publication Year :
- 2011
-
Abstract
- The need to simultaneously target infections with epidemiological overlap in the population with a single vaccine provides the basis for developing combination vaccines. Vibrio cholerae ghosts (rVCG) offer an attractive approach for developing vaccines against a number of human and animal pathogens. In this study, we constructed a multisubunit vaccine candidate co-expressing the serovar D-derived Porin B and polymorphic membrane protein-D proteins of Chlamydia trachomatis and evaluated its ability to simultaneously induce broad-based chlamydial immunity and elicit a vibriocidal antibody response to the Vibrio carrier envelope. Intramuscular (IM) immunization with the vaccine candidate elicited high levels of antigen-specific genital mucosal and systemic Th1 cell-mediated and humoral immune responses against heterologous serovars and strains, including serovars E-H and L. Also, in addition to the multisubunit vaccine, the single subunit constructs conferred significant cross protection against the heterologous mouse strain, Chlamydia muridarum. Furthermore, all mice immunized with rVCG vaccine constructs responded with a significant rise in vibriocidal antibody titer, the surrogate marker for protection in cholera. These findings demonstrate the ability of the multisubunit vaccine to induce cross protective chlamydial as well as vibriocidal immunity and establish the possibility of developing a broadly efficacious Chlamydia-cholera combination vaccine.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibody Formation
Bacterial Vaccines immunology
Chlamydia Infections immunology
Chlamydia trachomatis immunology
Cholera immunology
Cross Protection
Cytokines immunology
Female
Genetic Vectors
Membrane Proteins immunology
Mice
Mice, Inbred C57BL
Vaccines, Combined administration & dosage
Vaccines, Combined immunology
Vaccines, Subunit administration & dosage
Vaccines, Subunit immunology
Vibrio cholerae immunology
Antibodies, Bacterial blood
Bacterial Proteins immunology
Bacterial Vaccines administration & dosage
Chlamydia Infections prevention & control
Cholera prevention & control
Drug Evaluation
Porins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 29
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 21421002
- Full Text :
- https://doi.org/10.1016/j.vaccine.2011.03.027