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Rhizobium taibaishanense sp. nov., isolated from a root nodule of Kummerowia striata.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2012 Feb; Vol. 62 (Pt 2), pp. 335-341. Date of Electronic Publication: 2011 Mar 18. - Publication Year :
- 2012
-
Abstract
- During a study of the diversity and phylogeny of rhizobia in the root nodules of Kummerowia striata grown in north-western China, four strains were classified in the genus Rhizobium on the basis of their 16S rRNA gene sequences. The 16S rRNA gene sequences of three of these strains were identical and that of the other strain, which was the only one isolated in Yangling, differed from the others by just 1 bp. The16S rRNA gene sequences of the four strains showed a mean similarity of 99.3 % with the most closely related, recognized species, Rhizobium vitis. The corresponding recA and glnA gene sequences showed similarities with established species of Rhizobium of less than 86.5 % and less than 89.6 %, respectively. These low similarities indicated that the four strains represented a novel species of the genus Rhizobium. The strains were also found to be distinguishable from the closest related, established species (R. vitis) by rep-PCR DNA fingerprinting, analysis of cellular fatty acid profiles and from the results of a series of phenotypic tests. The level of DNA-DNA relatedness between the representative strain CCNWSX 0483(T) and Rhizobium vitis IAM 14140(T) was only 40.13 %. Therefore, a novel species, Rhizobium taibaishanense sp. nov., is proposed, with strain CCNWSX 0483(T) ( = ACCC 14971(T) = HAMBI 3214(T)) as the type strain. In nodulation and pathogenicity tests, none of the four strains of Rhizobium taibaishanense sp. nov. was able to induce any nodule or tumour formation on plants. As no amplicons were detected when DNA from the strains was run in PCR with primers for the detection of nodA, nifH and virC gene sequences, the strains probably do not carry sym or vir genes.
- Subjects :
- Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial analysis
Fatty Acids analysis
Glutamate-Ammonia Ligase genetics
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S genetics
Rec A Recombinases genetics
Rhizobium genetics
Rhizobium metabolism
Sequence Analysis, DNA
Species Specificity
Fabaceae microbiology
Plant Roots microbiology
Rhizobium classification
Rhizobium isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 62
- Issue :
- Pt 2
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 21421926
- Full Text :
- https://doi.org/10.1099/ijs.0.029108-0