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Suitability of different glycoproteins and test systems for detecting cross-reactive carbohydrate determinant-specific IgE in hymenoptera venom-allergic patients.
- Source :
-
International archives of allergy and immunology [Int Arch Allergy Immunol] 2011; Vol. 156 (1), pp. 43-50. Date of Electronic Publication: 2011 Mar 29. - Publication Year :
- 2011
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Abstract
- Background: In hymenoptera venom allergy, about 75% of detected in vitro double positivity to yellow jacket and honeybee venom is ascribed to specific IgE (sIgE) directed against cross-reactive carbohydrate determinants (CCDs). To date, for the detection of CCD-sIgE, different carbohydrate antigens and methods are used. The most suitable one still has to be identified.<br />Methods: Eighty-seven patients with confirmed hymenoptera venom allergy and venom sIgE values of ≥0.7 kU/l were investigated. Sixty-five patients showed sIgE reactivity to both yellow jacket and honeybee venom, 22 were venom mono positive and served as controls. Occurrence of CCD-sIgE was determined using bromelain, horseradish peroxidase (HRP) and MUXF(3) on system A, and ascorbic acid oxidase (AAO), bromelain and HRP on system B. Further, a reference standard for CCD-sIgE evaluation was created: CCD positivity was assumed when at least 4 of the 6 test results were positive.<br />Results: According to the defined reference standard, 45/65 venom double positive patients exhibited CCD-sIgE. Using system A, comparison with the reference standard revealed sensitivity and specificity values of 96 and 97%, respectively, for MUXF(3), 100 and 100%, respectively, for bromelain, and 96 and 97%, respectively, for HRP. Using system B, sensitivity and specificity was 98 and 97%, respectively, for AAO, 62 and 95%, respectively, for bromelain, and 96 and 69%, respectively, for HRP. Results of the 3 test allergens obtained with system A showed strong correlations (r = 0.932-0.976), whereas results with system B showed lower correlations (r = 0.714-0.898).<br />Conclusions: All 3 test allergens used with system A are suitable for CCD-sIgE detection in hymenoptera venom allergy. With system B, only AAO seems to be a reliable tool.<br /> (Copyright © 2011 S. Karger AG, Basel.)
- Subjects :
- Allergens chemistry
Allergens immunology
Animals
Bee Venoms chemistry
Bromelains chemistry
Bromelains immunology
Carbohydrate Sequence
Carbohydrates chemistry
Cross Reactions
Female
Horseradish Peroxidase chemistry
Humans
Hymenoptera immunology
Hypersensitivity, Immediate immunology
Male
Molecular Sequence Data
Polysaccharides chemistry
Polysaccharides immunology
Skin Tests
Wasp Venoms chemistry
Antibody Specificity
Bee Venoms immunology
Carbohydrates immunology
Glycoproteins chemistry
Glycoproteins immunology
Horseradish Peroxidase immunology
Hypersensitivity, Immediate diagnosis
Immunoglobulin E blood
Wasp Venoms immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0097
- Volume :
- 156
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International archives of allergy and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 21447958
- Full Text :
- https://doi.org/10.1159/000322279