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Structure of the glycosyl-phosphatidylinositol membrane anchor of the Leishmania major promastigote surface protease.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 1990 Oct 05; Vol. 265 (28), pp. 16955-64. - Publication Year :
- 1990
-
Abstract
- In common with many other plasma membrane glycoproteins of eukaryotic origin, the promastigote surface protease (PSP) of the protozoan parasite Leishmania contains a glycosyl-phosphatidylinositol (GPI) membrane anchor. The GPI anchor of Leishmania major PSP was purified following proteolysis of the PSP and analyzed by two-dimensional 1H-1H NMR, compositional and methylation linkage analyses, chemical and enzymatic modifications, and amino acid sequencing. From these results, the structure of the GPI-containing peptide was found to be Asp-Gly-Gly-Asn-ethanolamine-PO4-6Man alpha 1-6Man alpha 1-4GlcN alpha 1-6myo-inositol-1-PO4-(1-alkyl-2-acyl-glycerol). The glycan structure is identical to the conserved glycan core regions of the GPI anchor of Trypanosoma brucei variant surface glycoprotein and rat brain Thy-1 antigen, supporting the notion that this portion of GPIs are highly conserved. The phosphatidylinositol moiety of the PSP anchor is unusual, containing a fully saturated, unbranched 1-O-alkyl chain (mainly C24:0) and a mixture of fully saturated unbranched 2-O-acyl chains (C12:0, C14:0, C16:0, and C18:0). This lipid composition differs significantly from those of the GPIs of T. brucei variant surface glycoprotein and mammalian erythrocyte acetylcholinesterase but is similar to that of a family of glycosylated phosphoinositides found uniquely in Leishmania.
- Subjects :
- Amino Acid Sequence
Animals
Carbohydrate Conformation
Carbohydrate Sequence
Cell Membrane enzymology
Endopeptidases biosynthesis
Glycolipids isolation & purification
Glycosylphosphatidylinositols
Leishmania tropica genetics
Magnetic Resonance Spectroscopy
Methylation
Molecular Sequence Data
Myristic Acid
Myristic Acids metabolism
Phosphatidylinositols isolation & purification
Sequence Homology, Nucleic Acid
Trypanosoma brucei brucei genetics
Variant Surface Glycoproteins, Trypanosoma genetics
Endopeptidases genetics
Glycolipids biosynthesis
Leishmania tropica enzymology
Phosphatidylinositols biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 265
- Issue :
- 28
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 2145267