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New insight into molecular phylogeny and epidemiology of Sporothrix schenckii species complex based on calmodulin-encoding gene analysis of Italian isolates.
- Source :
-
Mycopathologia [Mycopathologia] 2011 Sep; Vol. 172 (3), pp. 179-86. Date of Electronic Publication: 2011 Apr 02. - Publication Year :
- 2011
-
Abstract
- In this study, we investigated phylogenetic relationships among Italian Sporothrix schenckii isolates, by comparing their partial calmodulin sequences. In this analysis, we used 26 environmental strains of S. schenckii, plus two autochthonous clinical isolates. The results showed that our clinical strains grouped with S. schenckii sensu stricto isolates, whereas all 26 environmental isolates co-clustered with Sporothrix albicans (now regarded as a synonym of Sporothrix pallida), a non-pathogenic species closely related to S. schenckii. Furthermore, the group of environmental strains was found to be quite heterogeneous and further subdivided into two subgroups. The data reported here also showed that molecular methods, for specific identification of S. schenckii, developed before the description of its closely related species should be used with caution because of the possibility of false positive results, which could lead to inappropriate antifungal therapy. This study improves our understanding of the distribution of these new closely related Sporothrix species which also showed significant differences in antifungal susceptibilities.
- Subjects :
- Cluster Analysis
DNA, Fungal chemistry
DNA, Fungal genetics
Genetic Variation
Genotype
Humans
Italy epidemiology
Molecular Epidemiology
Molecular Sequence Data
Sequence Analysis, DNA
Sporothrix isolation & purification
Calmodulin genetics
Environmental Microbiology
Phylogeny
Sporothrix classification
Sporothrix genetics
Sporotrichosis epidemiology
Sporotrichosis microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-0832
- Volume :
- 172
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Mycopathologia
- Publication Type :
- Academic Journal
- Accession number :
- 21461774
- Full Text :
- https://doi.org/10.1007/s11046-011-9420-z