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Novel changes in glycosylation of serum Apo-J in patients with hepatocellular carcinoma.
- Source :
-
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology [Cancer Epidemiol Biomarkers Prev] 2011 Jun; Vol. 20 (6), pp. 1222-9. Date of Electronic Publication: 2011 Apr 05. - Publication Year :
- 2011
-
Abstract
- Background: Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide and the occurrence of HCC has more than doubled in the United States in the past decade. Early detection is considered key to reducing the mortality of HCC.<br />Methods: Using two-dimensional gel electrophoresis and high-performance liquid chromatography we have analyzed the glycosylation of Apo-J from healthy controls, patients with liver cirrhosis, or those with HCC.<br />Results: Apo-J in the serum from patients with HCC had decreased levels of (β-1,4) triantennary N-linked glycan compared with the healthy controls or patients with liver cirrhosis. We analyzed this change in an independent cohort of 76 patients with HCC, 32 with cirrhosis, and 43 infected with hepatitis C virus using the Datura stramonium lectin (DSL), which binds to (β-1,4) triantennary N-linked glycan. The level of DSL-reactive Apo-J allowed us to differentiate HCC from cirrhosis with an area under the receiver operating characteristic curve (AUROC) of 0.852. When Apo-J was combined with other serum biomarkers such as α-fetoprotein (AFP) and fucosylated kininogen by using a multivariate logistic regression model, the AUROC increased to 0.944, a value much greater than that observed with AFP alone (AUROC of 0.765).<br />Conclusions: The glycosylation of Apo-J is a useful marker when used alone or in combination with outer makers for the early detection of HCC.<br />Impact: The potential use of a combination of AFP, DSL-reactive Apo-J, and fucosylated kininogen as a biomarker of HCC would have great value in the management of patients with liver disease.<br /> (©2011 AACR.)
- Subjects :
- Carcinoma, Hepatocellular blood
Case-Control Studies
Chromatography, Liquid
Electrophoresis, Gel, Two-Dimensional
Female
Glycosylation
Humans
Liver Cirrhosis blood
Liver Neoplasms blood
Male
Middle Aged
Neoplasm Staging
Polysaccharides analysis
ROC Curve
Risk Factors
Tandem Mass Spectrometry
alpha-Fetoproteins metabolism
Biomarkers, Tumor blood
Carcinoma, Hepatocellular diagnosis
Clusterin blood
Liver Cirrhosis diagnosis
Liver Neoplasms diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1538-7755
- Volume :
- 20
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
- Publication Type :
- Academic Journal
- Accession number :
- 21467232
- Full Text :
- https://doi.org/10.1158/1055-9965.EPI-10-1047