Back to Search
Start Over
Delayed re-epithelialization in periostin-deficient mice during cutaneous wound healing.
- Source :
-
PloS one [PLoS One] 2011 Apr 07; Vol. 6 (4), pp. e18410. Date of Electronic Publication: 2011 Apr 07. - Publication Year :
- 2011
-
Abstract
- Background: Matricellular proteins, including periostin, are important for tissue regeneration.<br />Methods and Findings: Presently we investigated the function of periostin in cutaneous wound healing by using periostin-deficient ⁻/⁻ mice. Periostin mRNA was expressed in both the epidermis and hair follicles, and periostin protein was located at the basement membrane in the hair follicles together with fibronectin and laminin γ2. Periostin was associated with laminin γ2, and this association enhanced the proteolytic cleavage of the laminin γ2 long form to produce its short form. To address the role of periostin in wound healing, we employed a wound healing model using WT and periostin⁻/⁻ mice and the scratch wound assay in vitro. We found that the wound closure was delayed in the periostin⁻/⁻ mice coupled with a delay in re-epithelialization and with reduced proliferation of keratinocytes. Furthermore, keratinocyte proliferation was enhanced in periostin-overexpressing HaCaT cells along with up-regulation of phosphorylated NF-κB.<br />Conclusion: These results indicate that periostin was essential for keratinocyte proliferation for re-epithelialization during cutaneous wound healing.
- Subjects :
- Animals
Basement Membrane metabolism
Blotting, Western
Cell Adhesion Molecules genetics
Cell Line
Epidermal Cells
Epidermis metabolism
Fibronectins metabolism
Hair Follicle cytology
Hair Follicle metabolism
Humans
Immunohistochemistry
Immunoprecipitation
In Situ Hybridization
Laminin genetics
Laminin metabolism
Mice
NF-kappa B genetics
NF-kappa B metabolism
Phosphorylation
Protein Binding
Reverse Transcriptase Polymerase Chain Reaction
Skin injuries
Wound Healing genetics
Cell Adhesion Molecules metabolism
Skin cytology
Skin metabolism
Wound Healing physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 6
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 21490918
- Full Text :
- https://doi.org/10.1371/journal.pone.0018410