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Interleukin-1 in lipopolysaccharide induced chorioamnionitis in the fetal sheep.

Authors :
Berry CA
Nitsos I
Hillman NH
Pillow JJ
Polglase GR
Kramer BW
Kemp MW
Newnham JP
Jobe AH
Kallapur SG
Source :
Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2011 Nov; Vol. 18 (11), pp. 1092-102. Date of Electronic Publication: 2011 Apr 14.
Publication Year :
2011

Abstract

We tested the hypothesis that interleukin 1 (IL-1) mediates intra-amniotic lipopolysaccharide (LPS)-induced chorioamnionitis in preterm fetal sheep. Time-mated Merino ewes with singleton fetuses received IL-1α, LPS, or saline (control) by intra-amniotic injection 1 to 2 days before operative delivery at 124 ± 1 days gestational age (N = 5-9/group; term = 150 days). Recombinant human IL-1 receptor antagonist (rhIL-1ra) was given into the amniotic fluid 3 hours before intra-amniotic LPS or saline to block IL-1 signaling. Inflammation in the chorioamnion was determined by histology, cytokine messenger RNA (mRNA), protein expression, and by quantitation of activated inflammatory cells. Intra-amniotic IL-1 and LPS both induced chorioamnionitis. However, IL-1 blockade with IL-1ra did not decrease intra-amniotic LPS-induced increases in pro-inflammatory cytokine mRNAs, numbers of inflammatory cells, myeloperoxidase, or monocyte chemotactic protein-1-expressing cells in the chorioamnion. We conclude that IL-1 and LPS both can cause chorioamnionitis, but IL-1 is not an important mediator of LPS-induced chorioamnionitis in fetal sheep.

Details

Language :
English
ISSN :
1933-7205
Volume :
18
Issue :
11
Database :
MEDLINE
Journal :
Reproductive sciences (Thousand Oaks, Calif.)
Publication Type :
Academic Journal
Accession number :
21493953
Full Text :
https://doi.org/10.1177/1933719111404609