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[Effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A expression in rat offspring].
- Source :
-
Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics [Zhongguo Dang Dai Er Ke Za Zhi] 2011 Apr; Vol. 13 (4), pp. 326-30. - Publication Year :
- 2011
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Abstract
- Objective: To study the effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A (PDGF-A) expression in rat offspring.<br />Methods: Sprague-Dawley (SD) female rats were randomly divided into two groups: normal control and vitamin D deficiency, with 6 rats in each group. The vitamin D deficiecy group was kept away from light and fed with the forage without vitamin D. After 2 weeks, the rats were mated with normal SD male rats. The morphological changes of fetal rat lungs on day 20 of gestation and 1-day-old neonatal rat lungs were observed by light microscope and electronic microscope. The levels of PDGF-A mRNA and protein in fetal and neonatal rat lungs were measured by reverse transcriptase-polymerase chain reaction (RT-PCR) technique and Western blot method respectively.<br />Results: Under the light microscope, smaller alveolar space, smaller diameter of the respiratory membrane and thicker alveolus mesenchyma were observed in lungs of fetal and neonatal rats from the vitamin D deficiency group compared with the controls (P<0.05). Under the electronic microscope, fewer lamellar bodies but more glycogen deposition in intracytoplasm were observed in the lungs of fetal rats from the vitamin D deficiency group compared with the controls. There was an increased number of empty lamellar bodies in neonatal rats from the vitamin D deficiency group. The levels of PDGF-A mRNA and protein in lungs of fetal and neonatal rats from the vitamin D deficiency group were significantly lower than the controls (P<0.05).<br />Conclusions: Maternal vitamin D deficiency during pregnancy may inhibit the development of lung morphogenesis and PDGF-A expression in late fetal and neonatal rats. The low expression of PDGF-A may be involved in the inhibitory effect of vitamin D deficiency on the lung development.
- Subjects :
- Animals
Calcifediol blood
Female
Lung pathology
Lung ultrastructure
Platelet-Derived Growth Factor analysis
Platelet-Derived Growth Factor metabolism
Pregnancy
RNA, Messenger analysis
Rats
Rats, Sprague-Dawley
Vitamin D Deficiency metabolism
Lung embryology
Platelet-Derived Growth Factor genetics
Vitamin D Deficiency embryology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1008-8830
- Volume :
- 13
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
- Publication Type :
- Academic Journal
- Accession number :
- 21507305