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[Effect of shRNAmir lentiviral vector-mediated COX-2 silencing on the radiosensitivity of nasopharyngeal carcinoma cells].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2011 Apr; Vol. 31 (4), pp. 599-603. - Publication Year :
- 2011
-
Abstract
- Objective: To evaluate the effect of COX-2 silencing on the radiosensitivity of a nasopharyngeal carcinoma (NPC) cell line C666-1.<br />Methods: Anti-COX-2 C666-1 cell line with COX-2 gene silencing mediated by shRNAmir lentiviral vector and the control cell line Anti-GL-2 C666-1 were exposed to various radiation doses. The clonogenic survival assay and curve fitting was used to calculate the radiobiological parameters and the sensitization enhancement ratio after the radiation. Cell cycle changes were assessed after the exposure by flow cytometric analysis. In a BALB/c nude mouse model, the growth curve of the xenografts was generated and the tumor growth inhibition rate was calculated.<br />Results: Compared with the control cells, Anti-COX-2 C666-1 cells showed obviously lowered values of SF2, D0 and Dq but significantly increased α/β with a sensitivity enhancement ratio of 1.4014. COX-2 gene silencing increased the inhibition rate of the tumor xenografts after the radiation, and caused also decreased percentage of G2/M arrest resulting from the exposure.<br />Conclusion: Stable COX-2 silencing in NPC cells can improve the effect of radiotherapy both in vitro and in vivo. By changing the radiobiological parameters, genetically based COX-2 inhibitor may be a potentially promising radiosensitizer of NPC.
- Subjects :
- Animals
Carcinoma
Cell Line, Tumor
Cell Survival
Female
Genetic Vectors
Humans
Lentivirus genetics
Mice
Mice, Inbred BALB C
Mice, Nude
Nasopharyngeal Carcinoma
Xenograft Model Antitumor Assays
Cyclooxygenase 2 genetics
Gene Silencing
Nasopharyngeal Neoplasms radiotherapy
RNA, Small Interfering
Radiation Tolerance genetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 31
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 21515451