Back to Search
Start Over
Dietary zerumbone prevents mouse cornea from UVB-induced photokeratitis through inhibition of NF-κB, iNOS, and TNF-α expression and reduction of MDA accumulation.
- Source :
-
Molecular vision [Mol Vis] 2011 Apr 06; Vol. 17, pp. 854-63. Date of Electronic Publication: 2011 Apr 06. - Publication Year :
- 2011
-
Abstract
- Purpose: Ultraviolet B (UVB) irradiation activates nuclear factor-kappa B (NF-κB) and inducible nitric oxide synthase (iNOS) in the cornea, resulting in inflammatory responses and malondialdehyde (MDA) accumulation. This study aims to determine the effect of zerumbone, a potent NF-κB inhibitor and inflammation modulators, on UVB-induced corneal damages in a mouse model.<br />Methods: Fifty female imprinting control region (ICR) mice were randomly divided into five groups. The mice were anaesthetized with their ocular surfaces exposed to UVB light (0.72J/cm(2)/daily), followed by daily dietary zerumbone supplements at 0, 1, 10, and 100 mg/kg of bodyweight. Mice without zerumbone supplements were used as treatment controls and mice without UVB irradiation as blank controls. Corneal surface damages were graded according to smoothness, opacity, and the extent of lissamine green staining. Histopathological changes were also examined, along with the expression of NF-κB, iNOS, and tumor necrosis factor-α (TNF-α). MDA accumulation and the levels of two antioxidant enzymes, glutathione (GSH) and GSH reductase (GR) were also examined.<br />Results: UVB irradiation caused significant damages to cornea, including sustained inflammation, apparent corneal ulcer, and severe epithelial exfoliation, leading to thinning of corneal epithelial layer, and infiltration of polymorphonuclear leukocytes. NF-κB expression was highly activated with nuclear translocation. The expression of iNOS and TNF-α were increased. MDA accumulation was also increased in both the corneal epithelial layer and the stroma. With dietary zerumbone, corneal damages were ameliorated in a dose-dependent manner. NF-κB activation and its nuclear translocation were blocked with decreased expression of iNOS and TNF-α. Infiltration of polymorphonuclear leukocytes was also blocked by dietary zerumbone. Besides, MDA accumulation was reduced with concomitant increase of GSH and GR levels.<br />Conclusions: Dietary zerumbone prevents UVB-induced corneal damages by inhibition of NF-κB, iNOS, and TNF-α, with concomitant reduction of MDA accumulation and increase of GSH and GR levels in the mouse model. Results of this study suggest that dietary zerumbone may be used as a prophylactic agent against UVB-induced photokeratitis.
- Subjects :
- Animals
Cornea pathology
Cornea radiation effects
Corneal Topography
Dose-Response Relationship, Drug
Female
Gene Expression
Glutathione analysis
Glutathione biosynthesis
Glutathione Reductase analysis
Keratitis etiology
Keratitis genetics
Keratitis metabolism
Keratitis pathology
Lissamine Green Dyes analysis
Malondialdehyde analysis
Mice
Mice, Inbred Strains
Models, Animal
NF-kappa B genetics
NF-kappa B metabolism
Neutrophil Infiltration drug effects
Nitric Oxide Synthase Type II genetics
Nitric Oxide Synthase Type II metabolism
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha metabolism
Ultraviolet Rays adverse effects
Cornea drug effects
Diet Therapy methods
Keratitis diet therapy
NF-kappa B antagonists & inhibitors
Nitric Oxide Synthase Type II antagonists & inhibitors
Sesquiterpenes administration & dosage
Sesquiterpenes therapeutic use
Tumor Necrosis Factor-alpha antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 1090-0535
- Volume :
- 17
- Database :
- MEDLINE
- Journal :
- Molecular vision
- Publication Type :
- Academic Journal
- Accession number :
- 21527993