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The GLN3 gene product is required for transcriptional activation of allantoin system gene expression in Saccharomyces cerevisiae.
- Source :
-
Journal of bacteriology [J Bacteriol] 1990 Feb; Vol. 172 (2), pp. 1014-8. - Publication Year :
- 1990
-
Abstract
- We show that mutation at the GLN3 locus results in decreased steady-state levels of DAL7, DUR1,2, CAR1, and URA3 mRNAs derived from cultures grown in the presence of inducer. Basal levels of these RNA species, however, were not significantly affected by a gln3 mutation. The GLN3 product appears to affect gene expression in two ways. The pleiotropic requirement of GLN3 for induced gene expression probably derives from the need of the GLN3 product for inducer uptake into the cell and its loss in gln3 mutants. We also demonstrate that transcriptional activation, mediated by the DAL5 and DAL7 upstream activation sequences, requires a functional GLN3 gene product. This observation identified transcriptional activation as the most likely point of GLN3 participation in the expression of allantoin system genes.
- Subjects :
- Base Sequence
DNA Transposable Elements
Genotype
Molecular Sequence Data
Nucleic Acid Hybridization
Plasmids
RNA, Messenger genetics
Restriction Mapping
Allantoin biosynthesis
Gene Expression Regulation, Fungal
Genes, Fungal
Genes, Regulator
Saccharomyces cerevisiae genetics
Transcription, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 172
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 2153652
- Full Text :
- https://doi.org/10.1128/jb.172.2.1014-1018.1990