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Immunohistochemical localization of renal mineralocorticoid receptor by using an anti-idiotypic antibody that is an internal image of aldosterone.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1990 Feb; Vol. 87 (3), pp. 1086-8. - Publication Year :
- 1990
-
Abstract
- A monoclonal antibody (H10E), generated by an auto-anti-idiotypic procedure and directed at the aldosterone-binding site of mineralocorticoid receptor (MR), was used in immunohistochemical studies to localize MR in rabbit kidney preparations. In agreement with earlier physiological and biochemical observations, MR was detected in connecting and cortical collecting tubules. Additionally, MR was detected in the distal tubules, the medullary and papillary collecting ducts, and in the epithelial cells lining the papilla. The internal image properties of the antibody were exploited to assess the specificity of MR detection by means of competition studies with hormones and antihormones. Immunostaining was completely abolished by preincubation with aldosterone but not with RU 486, a steroid antagonist that does not bind MR. On the cellular level, immunostaining occurred in the cytoplasm and, in the majority of cells, in the nucleus as well. The nucleocytoplasmic distribution of MR was unaffected by adrenalectomy or by administration of aldosterone. The availability of this specific monoclonal antibody makes it feasible to study MR expression in other target tissues and in pathological disorders.
- Subjects :
- Adrenalectomy
Aldosterone pharmacology
Animals
Immunoenzyme Techniques
Kidney drug effects
Kidney metabolism
Mineralocorticoids analysis
Rabbits
Receptors, Mineralocorticoid
Receptors, Steroid immunology
Reference Values
Aldosterone immunology
Antibodies, Monoclonal
Immunoglobulin Idiotypes
Kidney cytology
Receptors, Steroid analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 87
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 2153968
- Full Text :
- https://doi.org/10.1073/pnas.87.3.1086