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MicroRNA signatures differentiate melanoma subtypes.
- Source :
-
Cell cycle (Georgetown, Tex.) [Cell Cycle] 2011 Jun 01; Vol. 10 (11), pp. 1845-52. Date of Electronic Publication: 2011 Jun 01. - Publication Year :
- 2011
-
Abstract
- Melanoma is an aggressive cancer that is highly resistance to therapies once metastasized. We studied microRNA (miRNA) expression in clinical melanoma subtypes and evaluated different miRNA signatures in the background of gain of function somatic and inherited mutations associated with melanoma. Total RNA from 42 patient derived primary melanoma cell lines and three independent normal primary melanocyte cell cultures was evaluated by miRNA array. MiRNA expression was then analyzed comparing subtypes and additional clinicopathologic criteria including somatic mutations. The prevalence and association of an inherited variant in a miRNA binding site in the 3'UTR of the KRAS oncogene, referred to as the KRAS-variant, was also evaluated. We show that seven miRNAs, miR-142-3p, miR-486, miR-214, miR-218, miR-362, miR-650 and miR-31, were significantly correlated with acral as compared to non-acral melanomas (p < 0.04). In addition, we discovered that the KRAS-variant was enriched in non-acral melanoma (25%), and that miR-137 under expression was significantly associated with melanomas with the KRAS-variant. Our findings indicate that miRNAs are differentially expressed in melanoma subtypes and that their misregulation can be impacted by inherited gene variants, supporting the hypothesis that miRNA misregulation reflects biological differences in melanoma.
- Subjects :
- 3' Untranslated Regions
Binding Sites genetics
Humans
Melanoma pathology
Mutation
Oligonucleotide Array Sequence Analysis
Proto-Oncogene Proteins p21(ras)
RNA, Neoplasm analysis
Melanoma classification
Melanoma genetics
MicroRNAs analysis
Proto-Oncogene Proteins genetics
ras Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1551-4005
- Volume :
- 10
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Cell cycle (Georgetown, Tex.)
- Publication Type :
- Academic Journal
- Accession number :
- 21543894
- Full Text :
- https://doi.org/10.4161/cc.10.11.15777