Miniaturized enzyme production and development of micro-assays for cellulolytic and xylanolytic enzymes.

Authors :: Lee YM
Lee H
Kim GH
Kim JJ
Source :: Journal of microbiological methods [J Microbiol Methods] 2011 Jul; Vol. 86 (1), pp. 124-7. Date of Electronic Publication: 2011 Apr 23.
Publication Year :: 2011
Abstract: Miniaturized fungal cultivation and enzyme assays were developed. Cultivation for enzyme production was performed in 50mL conical tubes. In addition, the miniaturized enzyme assays reduced the amount of enzymes and reagents necessary. These procedures can be adopted in screening fungi to determine if they produce cellulolytic and xylanolytic enzymes.<br /> (Copyright © 2011 Elsevier B.V. All rights reserved.)

Subjects :: Cellulase metabolism
Fungal Proteins metabolism
Fungi chemistry
Xylosidases metabolism
Cellulase analysis
Enzyme Assays methods
Fungal Proteins analysis
Fungi enzymology
Xylosidases analysis

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