Back to Search
Start Over
Selection of T-cell receptors with a recurrent CDR3β peptide-contact motif within the repertoire of alloreactive CD8(+) T cells.
- Source :
-
European journal of immunology [Eur J Immunol] 2011 Aug; Vol. 41 (8), pp. 2414-23. Date of Electronic Publication: 2011 Jun 24. - Publication Year :
- 2011
-
Abstract
- Peptide/MHC complexes recognized by alloreactive T lymphocytes (TLs) have been identified, but their contribution to in vivo allo-rejection is not known. We previously characterized the peptide pBM1, highly represented among endogenous H-2K(b) (K(b) )-associated peptides and critically required to induce full activation of H-2(k) monoclonal CD8(+) TLs expressing the cognate TCR-BM3.3. Here, we asked whether a pBM1/K(b) -specific TL subset could be detected within a polyclonal TL population rejecting allogeneic cells in vivo. We show that the proportion of pBM1/K(b) -binding CD8(+) TLs increased from <0.04% in naïve mice to 3% of activated CD44(+) CD8(+) TLs in H-2(k) mice rejecting K(b) -expressing cells. Among these, TCR-Vβ2 usage was greatly enriched, and 75% of them shared a TCR-Vβ2 CDR3β motif with the prototype TCR-BM3.3. Fewer than 5% of K(b) -reactive CD44(+) CD8(+) TLs not binding pBM1/K(b) displayed this CDR3β motif. We found that the recurrent CDR3β motif of pBM1/K(b) -binding TLs was assembled from distinct V/D/J recombination events, suggesting that it is recruited upon immunization for its optimal TCR-peptide/MHC fit. Thus, a CDR3β motif generated by a process akin to "convergent recombination" accounts for a sizable fraction of the alloreactive anti-K(b) TCR repertoire.<br /> (Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Amino Acid Motifs immunology
Amino Acid Sequence
Animals
CD8-Positive T-Lymphocytes metabolism
Complementarity Determining Regions genetics
Complementarity Determining Regions metabolism
Flow Cytometry
H-2 Antigens immunology
H-2 Antigens metabolism
Hyaluronan Receptors immunology
Hyaluronan Receptors metabolism
Immunization
Mice
Mice, Inbred CBA
Peptides metabolism
Protein Binding immunology
Receptors, Antigen, T-Cell genetics
Receptors, Antigen, T-Cell metabolism
Recombination, Genetic immunology
Sequence Analysis, DNA
VDJ Exons genetics
VDJ Exons immunology
CD8-Positive T-Lymphocytes immunology
Complementarity Determining Regions immunology
Peptides immunology
Receptors, Antigen, T-Cell immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1521-4141
- Volume :
- 41
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 21590766
- Full Text :
- https://doi.org/10.1002/eji.201141494