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Amphiphilic and biodegradable hy-PEI-g-PCL-b-PEG copolymers efficiently mediate transgene expression depending on their graft density.
- Source :
-
International journal of pharmaceutics [Int J Pharm] 2012 May 01; Vol. 427 (1), pp. 80-7. Date of Electronic Publication: 2011 May 12. - Publication Year :
- 2012
-
Abstract
- Novel biodegradable amphiphilic copolymers hy-PEI-g-PCL-b-PEG were prepared by grafting PCL-b-PEG chains onto hyper-branched poly(ethylene imine) as non-viral gene delivery vectors. Our investigations focused on the influence of graft densities of PCL-b-PEG chains on physico-chemical properties, DNA complexation and transfection efficiency. We found that the transfection efficiencies of these polymers increased at first towards an optimal graft density (n=3) and then decreased. The buffer-capacity-test showed almost exactly the same tendency as transfection efficiency. Cytotoxicity (MTT-assay) depended on the cooperation of PEG molecular weight and graft density of PCL-b-PEG chains. With increasing the graft density, cytotoxicity, zeta-potential, affinity with DNA, stability of the polyplexes and CMC-values were reduced strongly and regularly. Increasing the excess of polymer over DNA was shown to result in a decrease of the observed particle size to 100-200 nm.<br /> (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Subjects :
- Cell Line, Tumor
Drug Carriers administration & dosage
Drug Carriers toxicity
Ethylene Glycols administration & dosage
Humans
Inhibitory Concentration 50
Microscopy, Confocal
Particle Size
Plasmids chemistry
Polyesters administration & dosage
Polyethyleneimine administration & dosage
Drug Carriers chemistry
Ethylene Glycols chemistry
Gene Expression
Plasmids administration & dosage
Polyesters chemistry
Polyethyleneimine analogs & derivatives
Polyethyleneimine chemistry
Transfection
Transgenes
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3476
- Volume :
- 427
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of pharmaceutics
- Publication Type :
- Academic Journal
- Accession number :
- 21600970
- Full Text :
- https://doi.org/10.1016/j.ijpharm.2011.05.017