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A proteomic approach to analyze nitrogen- and cytokinin-responsive proteins in rice roots.
- Source :
-
Molecular biology reports [Mol Biol Rep] 2012 Feb; Vol. 39 (2), pp. 1617-26. Date of Electronic Publication: 2011 May 24. - Publication Year :
- 2012
-
Abstract
- Nitrogen plays a central role in rice growth and development because it modulates a wide variety of processes, including cytokinin (CK) metabolism. CK-mediated signaling is also related to nitrogen metabolism. The functional relation between nitrogen and CK are extremely complex and unclear. In this study, a comparative proteomic analysis was carried out to analyze proteins regulated by nitrogen and CK in rice roots. Proteins extracted from rice roots are separated by two-dimensional polyacrylamide gel electrophoresis. Thirty-two protein spots that expressed similarly by nitrogen and CK treatments are selected for identification by mass spectrometry. Of these spots, 28 are successfully identified. These proteins were categorized into classes related to energy, metabolism, disease/defense, protein degradation, signal transduction, transposons, and unclear classification. Energy gives the largest functional category, suggesting that the glycolysis (two enzymes detected) and tricarboxylic acid cycle (six enzymes detected) are accurately regulated by nitrogen and CK, thus promoting the synthesis of amino acid. The identification of novel proteins provides new insights into the coordination of nitrogen and CK in rice. The possible role of these proteins is discussed.
- Subjects :
- Analysis of Variance
Citric Acid Cycle genetics
Electrophoresis, Gel, Two-Dimensional
Enzyme-Linked Immunosorbent Assay
Glycolysis genetics
Mass Spectrometry
Oryza metabolism
Plant Roots metabolism
Cytokinins metabolism
Nitrogen metabolism
Oryza genetics
Plant Proteins genetics
Plant Proteins metabolism
Plant Roots genetics
Proteomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1573-4978
- Volume :
- 39
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular biology reports
- Publication Type :
- Academic Journal
- Accession number :
- 21607616
- Full Text :
- https://doi.org/10.1007/s11033-011-0901-4