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Genetic context and structural diversity of class 1 integrons from human commensal bacteria in a hospital intensive care unit.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2011 Aug; Vol. 55 (8), pp. 3939-43. Date of Electronic Publication: 2011 May 31. - Publication Year :
- 2011
-
Abstract
- Most surveys for class 1 integrons are at least partly predicated on PCR screening that targets integron conserved regions. However, class 1 integrons are structurally diverse, so dependence on conserved regions may lead to missing clinically relevant examples of class 1 integrons. Here, we surveyed a commensal population of bacteria from patients in an intensive care unit to identify class 1 integrons irrespective of their structure or genetic context. We identified several examples of class 1 integrons linked to complete Tn402-like or Tn402 hybrid transposition modules and diverse insertion points with respect to the inverted repeat IRi boundary. The diversity and abundance of class 1 integrons identified are such that many novel elements seen here would not have been identified by commonly used methods, and they revealed an additional level of complexity.
- Subjects :
- DNA, Bacterial chemistry
DNA, Bacterial genetics
Drug Resistance, Bacterial genetics
Enterobacteriaceae genetics
Humans
Klebsiella pneumoniae genetics
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Analysis, DNA
DNA Transposable Elements genetics
Gram-Negative Bacteria genetics
Integrons genetics
Intensive Care Units
Inverted Repeat Sequences genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1098-6596
- Volume :
- 55
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 21628540
- Full Text :
- https://doi.org/10.1128/AAC.01831-10