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Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells.
- Source :
-
Journal of biomedical science [J Biomed Sci] 2011 Jun 14; Vol. 18, pp. 39. Date of Electronic Publication: 2011 Jun 14. - Publication Year :
- 2011
-
Abstract
- Background: Stanniocalcin-1(STC-1) is up-regulated in several cancers including gastric cancer. Evidences suggest that STC-1 is associated with carcinogenesis and angiogenic process. However, it is unclear on the exact role for STC-1 in inducing angiogenesis and tumorigeneisis.<br />Method: BGC/STC cells (high-expression of STC-1) and BGC/shSTC cells (low- expression of STC-1) were constructed to investigate the effect of STC-1 on the xenograft tumor growth and angiogenesis in vitro and in vivo. ELISA assay was used to detect the expression of vascular endothelial growth factor (VEGF) in the supernatants. Neutralizing antibody was used to inhibit VEGF expression in supernatants. The expression of phosphorylated -PKCβII, phosphorylated -ERK1/2 and phosphorylated -P38 in the BGC treated with STC-1protein was detected by western blot.<br />Results: STC-1 could promote angiogenesis in vitro and in vivo, and the angiogenesis was consistent with VEGF expression in vitro. Inhibition of VEGF expression in supernatants with neutralizing antibody markedly abolished angiogenesis induced by STC-1 in vitro. The process of STC-1-regulated VEGF expression was mediated via PKCβII and ERK1/2.<br />Conclusions: STC-1 promotes the expression of VEGF depended on the activation of PKCβII and ERK1/2 pathways. VEGF subsequently enhances tumor angiogenesis which in turn promotes the gastric tumor growth.
- Subjects :
- Angiogenesis Inducing Agents pharmacology
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Glycoproteins genetics
Humans
Mitogen-Activated Protein Kinase 1 metabolism
Mitogen-Activated Protein Kinase 3 metabolism
Neovascularization, Pathologic pathology
Protein Kinase C metabolism
Protein Kinase C beta
Signal Transduction
Stomach Neoplasms metabolism
Stomach Neoplasms pathology
Tumor Cells, Cultured
Vascular Endothelial Growth Factor A pharmacology
Angiogenesis Inducing Agents metabolism
Endothelial Cells metabolism
Glycoproteins metabolism
Neovascularization, Pathologic metabolism
Stomach Neoplasms blood supply
Up-Regulation
Vascular Endothelial Growth Factor A metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0127
- Volume :
- 18
- Database :
- MEDLINE
- Journal :
- Journal of biomedical science
- Publication Type :
- Academic Journal
- Accession number :
- 21672207
- Full Text :
- https://doi.org/10.1186/1423-0127-18-39