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Cellulase and xylanase activity in relation to biofilm formation by two intertidal filamentous fungi in a novel polymethylmethacrylate conico-cylindrical flask.
- Source :
-
Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2011 Nov; Vol. 34 (9), pp. 1087-101. Date of Electronic Publication: 2011 Jun 17. - Publication Year :
- 2011
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Abstract
- A polymethylmethacrylate (PMMA) conico-cylindrical flask (CCF) with an inner arrangement consisting of eight equidistantly spaced rectangular strips mounted radially on a circular disk to provide additional surface area for fungal attachment was employed for production of cellulase by Chaetomium crispatum and xylanase by Gliocladium viride. The design allowed comparison of production between CCFs with hydrophobic surface (PMMA-CCF), hydrophilic glass surface (GS-CCF) and 500-ml Erlenmeyer flask (EF). Compared with the EF, endo-β-1,4-glucanase and FPase (filter paper degradation) activities increased from 0.044 to 0.156 and from 0.008 to 0.021 IU/ml, respectively, in the PMMA-CCF, while growth of C. crispatum was higher by at most 1.38-fold compared with the other vessels. Xylanase production in the EF was at most 5.08-fold higher and growth of G. viride was at most 1.52-fold higher compared with the other vessels. Temporal pattern of biofilm development based on two-channel fluorescence detection of extracellular polymeric substances (EPSs) and whole cells in a confocal laser scanning microscope demonstrated increase by 100% in biovolume, 25% in thickness and 62.5% both in substratum coverage and total spreading of C. crispatum biofilm in PMMA-CCF over 6 days. Biovolume of G. viride biofilm in GS-CCF increased by 150% over 4 days while that in PMMA-CCF enhanced by 200% over 2 days. Biofilm thickness in PMMA-CCF was 44% higher compared with GS-CCF and increased by 175% over 2 days. Substratum coverage was 38% higher in GS-CCF compared with PMMA-CCF. Thus, reactor surface area and property, shear forces and biofilm formation influenced enzyme production.
- Subjects :
- Biofilms
Bioreactors
Cellulose chemistry
Chaetomium metabolism
Equipment Design
Glass
Gliocladium metabolism
Hydrogen-Ion Concentration
Microscopy, Confocal methods
Plankton
Polymers chemistry
Surface Properties
Temperature
Time Factors
Cellulase analysis
Endo-1,4-beta Xylanases analysis
Fungi metabolism
Polymethyl Methacrylate chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1615-7605
- Volume :
- 34
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Bioprocess and biosystems engineering
- Publication Type :
- Academic Journal
- Accession number :
- 21681588
- Full Text :
- https://doi.org/10.1007/s00449-011-0559-2