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Plant polyphenols attenuate hepatic injury after hemorrhage/resuscitation by inhibition of apoptosis, oxidative stress, and inflammation via NF-kappaB in rats.
- Source :
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European journal of nutrition [Eur J Nutr] 2012 Apr; Vol. 51 (3), pp. 311-21. Date of Electronic Publication: 2011 Jun 23. - Publication Year :
- 2012
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Abstract
- Purpose: Oxidative stress and inflammation contribute to hepatic injury after hemorrhage/resuscitation (H/R). Natural plant polyphenols, i.e., green tea extract (GTE) possess high anti-oxidant and anti-inflammatory activities in various models of acute inflammation. However, possible protective effects and feasible mechanisms by which plant polyphenols modulate pro-inflammatory, apoptotic, and oxidant signaling after H/R in the liver remain unknown. Therefore, we investigated the effects of GTE and its impact on the activation of NF-kappaB in the pathogenesis of hepatic injury induced by H/R.<br />Methods: Twenty-four female LEWIS rats (180-250 g) were fed a standard chow (ctrl) or a diet containing 0.1% polyphenolic extracts (GTE) from Camellia sinensis starting 5 days before H/R. Rats were hemorrhaged to a mean arterial pressure of 30 ± 2 mmHg for 60 min and resuscitated (H/R and GTE H/R groups). Control groups (sham, ctrl, and GTE) underwent surgical procedures without H/R. Two hours after resuscitation, tissues were harvested.<br />Results: Plasma alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) increased 3.5-fold and fourfold, respectively, in vehicle-treated rats as compared to GTE-fed rats. Histopathological analysis revealed significantly decreased hepatic necrosis and apoptosis in GTE-fed rats after H/R. Real-time PCR showed that GTE diminished gene expression of pro-apoptotic caspase-8 and Bax, while anti-apoptotic Bcl-2 was increased after H/R. Hepatic oxidative (4-hydroxynonenal) and nitrosative (3-nitrotyrosine) stress as well as systemic IL-6 level and hepatic IL-6 mRNA were markedly reduced in GTE-fed rats compared with controls after H/R. Plant polyphenols also decreased the activation of both JNK and NFκB.<br />Conclusions: Taken together, GTE application blunts hepatic damage, apoptotic, oxidative, and pro-inflammatory changes after H/R. These results underline the important roles of JNK and NF-kappaB in inflammatory processes after H/R and the beneficial impact of plant polyphenols in preventing their activation.
- Subjects :
- Acute Disease
Alanine Transaminase blood
Aldehydes isolation & purification
Animals
Anti-Inflammatory Agents pharmacology
Antioxidants pharmacology
Caspase 8 genetics
Caspase 8 metabolism
Female
Gene Expression Regulation
Hemorrhage pathology
Inflammation pathology
Interleukin-6 blood
L-Lactate Dehydrogenase blood
Liver metabolism
Liver pathology
RNA isolation & purification
Rats
Rats, Inbred Lew
Resuscitation
Tea chemistry
Tyrosine analogs & derivatives
Tyrosine isolation & purification
bcl-2-Associated X Protein genetics
bcl-2-Associated X Protein metabolism
Apoptosis drug effects
Liver drug effects
NF-kappa B metabolism
Oxidative Stress drug effects
Plant Extracts pharmacology
Polyphenols pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1436-6215
- Volume :
- 51
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- European journal of nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 21698494
- Full Text :
- https://doi.org/10.1007/s00394-011-0216-1