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Nestin expression in Bowen's disease and Bowen's carcinoma associated with human papillomavirus.
- Source :
-
European journal of dermatology : EJD [Eur J Dermatol] 2011 Jul-Aug; Vol. 21 (4), pp. 515-9. - Publication Year :
- 2011
-
Abstract
- Human papillomaviruses (HPVs) have been detected in lesions of Bowen's disease (BD) and Bowen's carcinoma (BC); the invasive tumor retains the cytological characteristics of BD. Previous reports suggest that nestin-expressing hair follicle stem cells are undifferentiated and pluripotent, and nestin expression in some tumors indicates poor differentiation and high grade of malignancy. We identified HPV-DNA in BD (n=25) and BC (n=23) by in situ hybridization (ISH) analysis with INFORM(®) HPV III (Ventana Medical Systems. AZ, USA) and determined nestin expression by indirect immunohistochemical staining with anti-nestin polyclonal antibody (IBL, Gunma, Japan). We detected HPV-DNA in 68% of BD and in 87% of BC. In BD, 13 cases demonstrated the punctuate pattern, and four showed nestin expression. In BC, 19 cases showed the punctuate pattern and 16 showed nestin expression. HPV-DNA integrates into the host genome, and this is observed as the punctuate pattern on ISH. The nestin expression was statistically high in group of BC than BD (P<0.01). These results therefore suggest that HPV-DNA integrated in the genome of tumor cells of these diseases and contributed to malignant alteration. From the standpoint of tumorigenesis, BC might represent one type of poorly differentiated, high-grade squamous cell carcinoma.
- Subjects :
- Adult
Aged
Aged, 80 and over
DNA, Viral analysis
Female
Humans
Immunoenzyme Techniques
In Situ Hybridization
Male
Middle Aged
Nestin
Papillomaviridae isolation & purification
Bowen's Disease metabolism
Bowen's Disease virology
Carcinoma, Squamous Cell metabolism
Carcinoma, Squamous Cell virology
Intermediate Filament Proteins metabolism
Nerve Tissue Proteins metabolism
Papillomavirus Infections metabolism
Papillomavirus Infections virology
Skin Neoplasms metabolism
Skin Neoplasms virology
Tumor Virus Infections metabolism
Tumor Virus Infections virology
Subjects
Details
- Language :
- English
- ISSN :
- 1167-1122
- Volume :
- 21
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- European journal of dermatology : EJD
- Publication Type :
- Academic Journal
- Accession number :
- 21700534
- Full Text :
- https://doi.org/10.1684/ejd.2011.1391