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[Effect of tanshinone II A on TLR4 and TNF-α of endothelial cells induced by LPS].
- Source :
-
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi] 2011 Jul; Vol. 27 (7), pp. 733-5. - Publication Year :
- 2011
-
Abstract
- Aim: To observe the influence of tanshinone II A on the expression of toll like receptor 4(TLR4) and tumor necrosis factor-α(TNF-α) in cultured human umbilical veins endothelial cells EA.hy926 induced by LPS, and to investigate the molecular mechanism of tanshinone II A against atherosclerosis(AS).<br />Methods: The EA.hy926 cells were cultured in vitro. The mRNA and protein expression of TLR4 were tested by RT-PCR and immunocytochemistry technique respectively. The mRNA and protein expression of TNF-α were detected by RT-PCR and ELISA technique respectively.<br />Results: The expression of TLR4 and TNF-α were both increased compared to normal group after stimulation of LPS(P<0.05). The expression of TLR4 and TNF-α in tanshinone II A group were obviously inhibited compared to stimulation group(P<0.05).<br />Conclusion: The molecular mechanism of tanshinone II A against AS is probably to inhibit the expression of TLR4 and TNF-α.
- Subjects :
- Abietanes therapeutic use
Atherosclerosis drug therapy
Cell Line
Gene Expression Regulation drug effects
Humans
RNA, Messenger genetics
RNA, Messenger metabolism
Abietanes pharmacology
Endothelial Cells drug effects
Endothelial Cells metabolism
Lipopolysaccharides pharmacology
Toll-Like Receptor 4 genetics
Tumor Necrosis Factor-alpha genetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1007-8738
- Volume :
- 27
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 21722522