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Dengue virus isolation relying on antibody-dependent enhancement mechanism using FcγR-expressing BHK cells and a monoclonal antibody with infection-enhancing capacity.
- Source :
-
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2011 Nov; Vol. 52 (3), pp. 225-30. Date of Electronic Publication: 2011 Aug 06. - Publication Year :
- 2011
-
Abstract
- Background: Virus isolation is the most reliable evidence of dengue virus (DENV) infection. However, conventional virus isolation methods generally posses lower sensitivity and are time consuming as compared to other diagnostic methods such as detection of viral genome by RT-PCR, and determination of NS1 antigen and anti-DENV antibody by ELISA.<br />Objectives: A virus isolation method relying on the antibody-dependent enhancement mechanism was established and the assay's efficacy in DENV isolation was confirmed.<br />Study Design: FcγR-expressing BHK cells were used for DENV isolation from patient serum samples in the presence of a flavivirus-group reactive monoclonal antibody, mAb4G2, which possesses DENV infection-enhancement activity. DENV genome copy numbers in the culture supernatant fluids of FcγR-expressing BHK cells were assessed and compared to those of parent BHK cells and C6/36 mosquito cells, a cell line commonly used for DENV isolation.<br />Results: The virus titer levels were higher in the culture supernatant fluid of FcγR-expressing BHK cells in the presence of enhancing antibody in comparison with other cell lines using laboratory-established strains and some clinical samples. DENV was isolated from 7 of 16 serum samples by using FcγR-expressing BHK cells in the presence of mAb4G2, but not by using cell lines commonly employed in conventional isolation assays, the FcγR-negative BHK cells and C6/36 cell lines.<br />Conclusions: The results demonstrate that FcγR-expressing BHK cell line in the presence of antibodies, which possess antibody dependent enhancement (ADE) activity, is a useful tool for DENV isolation.<br /> (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Antibodies, Blocking immunology
Antibodies, Monoclonal immunology
Antibodies, Viral blood
Cell Line
Cricetinae
Culicidae virology
Dengue virology
Dengue Virus genetics
Dengue Virus immunology
Humans
RNA, Viral blood
Sensitivity and Specificity
Viral Load
Antibodies, Viral immunology
Antibody-Dependent Enhancement
Dengue diagnosis
Dengue Virus isolation & purification
Immunoglobulin Fc Fragments immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-5967
- Volume :
- 52
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
- Publication Type :
- Academic Journal
- Accession number :
- 21824813
- Full Text :
- https://doi.org/10.1016/j.jcv.2011.07.009