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Proteome reference map and regulation network of neonatal rat cardiomyocyte.
- Source :
-
Acta pharmacologica Sinica [Acta Pharmacol Sin] 2011 Sep; Vol. 32 (9), pp. 1116-27. Date of Electronic Publication: 2011 Aug 15. - Publication Year :
- 2011
-
Abstract
- Aim: To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte.<br />Methods: Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Biological networks and pathways of the neonatal rat cardiomyocytes were analyzed using the Ingenuity Pathway Analysis (IPA) program (www.ingenuity.com). A 2-DE database was made accessible on-line by Make2ddb package on a web server.<br />Results: More than 1000 proteins were separated on 2D gels, and 148 proteins were identified. The identified proteins were used for the construction of an extensible markup language-based database. Biological networks and pathways were constructed to analyze the functions associate with cardiomyocyte proteins in the database. The 2-DE database of rat cardiomyocyte proteins can be accessed at http://2d.bjmu.edu.cn.<br />Conclusion: A proteome reference map and regulation network of the neonatal rat cardiomyocytes have been established, which may serve as an international platform for storage, analysis and visualization of cardiomyocyte proteomic data.
- Subjects :
- Animals
Cells, Cultured
Male
Metabolic Networks and Pathways
Proteome isolation & purification
Rats
Rats, Sprague-Dawley
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Electrophoresis, Gel, Two-Dimensional methods
Myocytes, Cardiac chemistry
Proteome analysis
Proteomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1745-7254
- Volume :
- 32
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Acta pharmacologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 21841810
- Full Text :
- https://doi.org/10.1038/aps.2011.86