Back to Search
Start Over
Identification of immunodominant epitopes of alpha-crystallins recognized by antibodies in sera of patients with uveitis.
- Source :
-
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie [Graefes Arch Clin Exp Ophthalmol] 2012 Feb; Vol. 250 (2), pp. 297-305. Date of Electronic Publication: 2011 Aug 13. - Publication Year :
- 2012
-
Abstract
- Background: A high incidence of autoantibodies to lens proteins has been found in sera of patients with uveitis. We showed previously that the anti-lens antibodies reacted predominantly with α-crystallins. The aim of the present study was to identify immunodominant epitopes within the protein chains of human αA- and αB-crystallin.<br />Methods: Epitope specificities of antibodies to αA- and αB-crystallin were examined by ELISA using synthetic overlapping peptides, spanning the entire length of both α-crystallins. The peptides consisted of 25 amino acid residues, with an overlap of at least eight amino acids each. The synthetic peptides were tested against sera of 110 patients with different uveitis forms, classified according to anatomical location of intraocular inflammation.<br />Results: Four immunodominant regions within the protein chains of αA- and αB-crystallin could be identified. These regions were recognized by antibodies in sera of 56% of uveitis patients. Anti-lens antibodies of IgG-type reacted preferentially with regions located at amino acid (aa) residues aa:69-93 and aa:137-161 of αA-crystallin as well as aa:69-110 and aa:137-161 of αB-crystallin. IgM antibodies recognized predominantly region aa:149-173 of αA-crystallin, and aa:69-110 and aa:151-175 of αB-crystallin. IgM antibodies directed to peptide aa:69-93 of αB-crystallin were found in sera of 30% of patients with intermediate uveitis.<br />Conclusions: Four immunodominant B-cell epitopes within the protein chains of αA- and αB-crystallin have been identified; however, no clear correlation with the anatomically defined uveitis subtypes has been found except for intermediate uveitis. Whether there may be a correlation with uveitis forms with similar etiopathogenesis has to be evaluated in further studies.
- Subjects :
- Adolescent
Adult
Aged
Amino Acid Sequence
Child
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Epitope Mapping
Female
Humans
Immunoglobulin G blood
Immunoglobulin M blood
Male
Middle Aged
Molecular Sequence Data
Peptide Fragments immunology
Autoantibodies blood
Autoantigens immunology
Immunodominant Epitopes immunology
Uveitis immunology
alpha-Crystallin A Chain immunology
alpha-Crystallin B Chain immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1435-702X
- Volume :
- 250
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
- Publication Type :
- Academic Journal
- Accession number :
- 21842131
- Full Text :
- https://doi.org/10.1007/s00417-011-1758-x