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Expression of macro non-coding RNAs Meg8 and Irm in mouse embryonic development.
- Source :
-
Acta histochemica [Acta Histochem] 2012 Jul; Vol. 114 (4), pp. 392-9. - Publication Year :
- 2012
-
Abstract
- Non-coding RNAs (ncRNAs) Meg8 and Irm were previously identified as alternatively splicing isoforms of Rian gene. Ascertaining ncRNAs spatiotemporal expression patterns is crucial for understanding the physiological roles of ncRNAs during tissue and organ development. In this study in mouse embryos, we focused on the developmental regulation expression of imprinted macro ncRNAs, Meg8 and Irm by using in situ hybridization and quantitative real-time RT-PCR (QRT-PCR). The in situ hybridization results showed that Meg8 and Irm were expressed in the developing brain at embryonic day 10.5 (E10.5) and E11.5, while Irm expression signals were strikingly detected in the somite, where Meg8 expression signals were undetectable. By E15.5, they were expressed in brain, tongue, liver, lung and neuroendocrine tissues, while Irm displayed more restricted expression in tongue and skeletal muscle than Meg8. Furthermore, quantitative analysis confirmed that they were highly expressed in tongue and brain at E12.5, E15.5 and E18.5. These results indicated that Meg8 and Irm might be coordinately expressed and functionally correlated in diverse of organs. Notably, Irm was more closely associated with morphogenesis of skeletal muscle in contrast to Meg8 during embryonic development.<br /> (Copyright © 2011 Elsevier GmbH. All rights reserved.)
- Subjects :
- Alternative Splicing
Animals
Brain embryology
Brain metabolism
Embryonic Development
Female
In Situ Hybridization
Mice
Mice, Inbred C57BL
Muscle, Skeletal embryology
Muscle, Skeletal metabolism
Nuclear Proteins metabolism
Organ Specificity
Pregnancy
RNA, Untranslated metabolism
Real-Time Polymerase Chain Reaction
Tongue embryology
Tongue metabolism
Gene Expression Regulation, Developmental
Genomic Imprinting
Nuclear Proteins genetics
RNA, Untranslated genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0372
- Volume :
- 114
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Acta histochemica
- Publication Type :
- Academic Journal
- Accession number :
- 21855964
- Full Text :
- https://doi.org/10.1016/j.acthis.2011.07.009