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Characterization of human melanoma cell lines and melanocytes by proteome analysis.
- Source :
-
Cell cycle (Georgetown, Tex.) [Cell Cycle] 2011 Sep 01; Vol. 10 (17), pp. 2924-36. Date of Electronic Publication: 2011 Sep 01. - Publication Year :
- 2011
-
Abstract
- We have analyzed the proteomes of two human melanoma cell lines (A375 and 526), and of the human melanocytes, (FOM 78), by two-dimensional electrophoresis (2D-PAGE) and liquid chromatography - tandem mass spectrometry (LC-MS/MS). Our comparative proteomic analysis revealed that six proteins were over-expressed in both melanoma cell lines as compared to melanocytes: galectin-1, inosine-5'-monophosphate dehydrogenase 2, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, protein DJ-1, cyclophilin A and cofilin-1. We show, for the first time, that only specific isoforms of these molecules are over-expressed in melanoma. Different protein profiles were also found between each individual melanoma cell line and the melanocytes. s-Methyl-5-thioadenosine phosphorylase, ubiquitin and ribosomal protein S27 a precursor, the basic form of protein DJ-1, annexin a1, proliferation associated protein 2g4, isoform alfa-enolase of alfa-enolase, protein disulfide-isomerase precursor, and elongation factor 2 were more strongly expressed in A375 cells compared to melanocytes. In 526 cells, 60s acidic ribosomal protein p1 and calreticulin precursor were more highly expressed than in melanocytes. These molecular differences may help in better understanding melanoma development and its different responsiveness to therapies. The identified proteins could be exploited as biomarkers or therapeutic targets for melanoma.<br /> (© 2011 Landes Bioscience)
- Subjects :
- Biomarkers
Blotting, Western
Cell Line, Tumor
Chromatography, Liquid
Cofilin 1 metabolism
Cyclophilin A metabolism
Electrophoresis, Gel, Two-Dimensional
Galectin 1 metabolism
Humans
IMP Dehydrogenase metabolism
Image Processing, Computer-Assisted
Intracellular Signaling Peptides and Proteins metabolism
Mass Spectrometry methods
Oncogene Proteins metabolism
Protein Deglycase DJ-1
Protein Isoforms metabolism
Proteomics
Melanocytes metabolism
Melanoma metabolism
Protein Precursors metabolism
Proteome analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1551-4005
- Volume :
- 10
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Cell cycle (Georgetown, Tex.)
- Publication Type :
- Academic Journal
- Accession number :
- 21857157
- Full Text :
- https://doi.org/10.4161/cc.10.17.17068