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Complete debromination of tetra- and penta-brominated diphenyl ethers by a coculture consisting of dehalococcoides and desulfovibrio species.
- Source :
-
Environmental science & technology [Environ Sci Technol] 2011 Oct 01; Vol. 45 (19), pp. 8475-82. Date of Electronic Publication: 2011 Sep 06. - Publication Year :
- 2011
-
Abstract
- Polybrominated diphenyl ethers (PBDEs) are widespread global contaminants due to their extensive usage as flame retardants. Among the 209 PBDE congeners, tetra-brominated diphenyl ether (tetra-BDE) (congener 47) and penta-BDEs (congeners 99 and 100) are the most abundant, toxic, and bioaccumulative congeners in the environment. However, little is known about microorganisms that carry out debromination of these congeners under anaerobic conditions. In this study, we describe a coculture GY2 consisting of Dehalococcoides and Desulfovibrio spp., which is capable of debrominating ∼1180 nM of congeners 47, 99, and 100 (88-100% removal) to the nonbrominated diphenyl ether at an average rate of 36.9, 19.8, and 21.9 nM day(-1), respectively. Ortho bromines are preferentially removed during the debromination process. The growth of Dehalococcoides links tightly with PBDE debromination, with an estimated growth yield of 1.99 × 10(14) cells per mole of bromide released, while the growth of Desulfovibrio could be independent of PBDEs. The growth-coupled debromination suggests that Dehalococcoides cells in the coculture GY2 are able to respire on PBDEs. Given the ubiquity and recalcitrance of the tetra- and penta-BDEs, complete debromination of these congeners to less toxic end products (e.g. diphenyl ether) is important for the restoration of PBDE-contaminated environments.
- Subjects :
- Biodegradation, Environmental
Biological Assay
Chloroflexi genetics
Chloroflexi growth & development
Coculture Techniques
Denaturing Gradient Gel Electrophoresis
Desulfovibrio genetics
Halogenated Diphenyl Ethers chemistry
Phylogeny
RNA, Ribosomal, 16S genetics
Time Factors
Chloroflexi metabolism
Desulfovibrio metabolism
Halogenated Diphenyl Ethers metabolism
Halogenation
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5851
- Volume :
- 45
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- Environmental science & technology
- Publication Type :
- Academic Journal
- Accession number :
- 21859110
- Full Text :
- https://doi.org/10.1021/es201559g