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Pleiotrophin triggers inflammation and increased peritoneal permeability leading to peritoneal fibrosis.

Authors :
Yokoi H
Kasahara M
Mori K
Ogawa Y
Kuwabara T
Imamaki H
Kawanishi T
Koga K
Ishii A
Kato Y
Mori KP
Toda N
Ohno S
Muramatsu H
Muramatsu T
Sugawara A
Mukoyama M
Nakao K
Source :
Kidney international [Kidney Int] 2012 Jan; Vol. 81 (2), pp. 160-9. Date of Electronic Publication: 2011 Aug 31.
Publication Year :
2012

Abstract

Long-term peritoneal dialysis induces peritoneal fibrosis with submesothelial fibrotic tissue. Although angiogenesis and inflammatory mediators are involved in peritoneal fibrosis, precise molecular mechanisms are undefined. To study this, we used microarray analysis and compared gene expression profiles of the peritoneum in control and chlorhexidine gluconate (CG)-induced peritoneal fibrosis mice. One of the 43 highly upregulated genes was pleiotrophin, a midkine family member, the expression of which was also upregulated by the solution used to treat mice by peritoneal dialysis. This growth factor was found in fibroblasts and mesothelial cells within the underlying submesothelial compact zones of mice, and in human peritoneal biopsy samples and peritoneal dialysate effluent. Recombinant pleiotrophin stimulated mitogenesis and migration of mouse mesothelial cells in culture. We found that in wild-type mice, CG treatment increased peritoneal permeability (measured by equilibration), increased mRNA expression of TGF-β1, connective tissue growth factor and fibronectin, TNF-α and IL-1β expression, and resulted in infiltration of CD3-positive T cells, and caused a high number of Ki-67-positive proliferating cells. All of these parameters were decreased in peritoneal tissues of CG-treated pleiotrophin-knockout mice. Thus, an upregulation of pleiotrophin appears to play a role in fibrosis and inflammation during peritoneal injury.

Details

Language :
English
ISSN :
1523-1755
Volume :
81
Issue :
2
Database :
MEDLINE
Journal :
Kidney international
Publication Type :
Academic Journal
Accession number :
21881556
Full Text :
https://doi.org/10.1038/ki.2011.305