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Quantification of pork, chicken and beef by using a novel reference molecule.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2011; Vol. 75 (9), pp. 1639-43. Date of Electronic Publication: 2011 Sep 07. - Publication Year :
- 2011
-
Abstract
- A standard plasmid was constructed as a novel reference molecule for use in real-time quantitative PCR assays to verify the identity of beef, pork, chicken, mutton, and horseflesh. The plasmid contained a target domain of the cytochrome b (cyt b) gene and an artificial DNA sequence. Primers CO-F and CO-R, and probe CO-P were specifically designed to detect the artificial sequence. The calculated R² values of the standard curves (10³-10⁷ copies per reaction) for the five species ranged between 0.998 and 0.999 in the quantification analysis. The constructed plasmid provides a universal method for measuring the copy number of cyt b DNA in minced meat. This method would be a useful procedure for verifying food labels.
- Subjects :
- Animals
Base Sequence
Cattle
Chickens
Cytochromes b chemistry
DNA Primers chemistry
DNA Probes chemistry
DNA Probes genetics
Escherichia coli
Gene Dosage
Genes, Synthetic
Horses
Molecular Sequence Data
Plasmids chemistry
Plasmids genetics
Sheep
Swine
Biological Assay
Cytochromes b genetics
DNA Primers genetics
DNA Probes analysis
Meat Products analysis
Plasmids analysis
Real-Time Polymerase Chain Reaction methods
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 75
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 21897035
- Full Text :
- https://doi.org/10.1271/bbb.110024