Back to Search
Start Over
Rapamycin affects tissue plasminogen activator and plasminogen activator inhibitor I expression: a potential prothrombotic mechanism of drug-eluting stents.
- Source :
-
Angiology [Angiology] 2012 Jul; Vol. 63 (5), pp. 330-5. Date of Electronic Publication: 2011 Sep 07. - Publication Year :
- 2012
-
Abstract
- Although drug-eluting stents (DESs) can decrease the risk of restenosis, this benefit is tempered by a possible increased risk of in-stent thrombosis. We assessed the effects of rapamycin on human umbilical vein endothelial cells (HUVECs) to identify the alterations in gene expression associated with thrombosis. Expression of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1) was assessed in HUVECs treated with rapamycin (final concentrations: 1, 10, 100, and 1000 ng/mL) for 24 and 48 hours. Incubation of HUVECs with rapamycin strongly reduced the expression of t-PA in a concentration-dependant manner (P < .05 to < .01). However, the expression of PAI-1 was induced by rapamycin (P < .05 to < .01). The increase in PAI-1 induction was up to 3.3-fold. In conclusion, rapamycin inhibited t-PA and induced PAI-1 expression in HUVECs. This effect may contribute to in-stent thrombosis associated with DESs.
- Subjects :
- Cells, Cultured
Dose-Response Relationship, Drug
Down-Regulation
Human Umbilical Vein Endothelial Cells metabolism
Humans
Plasminogen Activator Inhibitor 1 genetics
Prosthesis Design
RNA, Messenger metabolism
Thrombosis genetics
Thrombosis metabolism
Time Factors
Tissue Plasminogen Activator genetics
Up-Regulation
Cardiovascular Agents toxicity
Drug-Eluting Stents
Human Umbilical Vein Endothelial Cells drug effects
Plasminogen Activator Inhibitor 1 metabolism
Sirolimus toxicity
Thrombosis chemically induced
Tissue Plasminogen Activator metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1940-1574
- Volume :
- 63
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Angiology
- Publication Type :
- Academic Journal
- Accession number :
- 21900343
- Full Text :
- https://doi.org/10.1177/0003319711418219