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Preparation and biological evaluation of enzyme-assisted extracts from edible seaweed (Enteromorpha prolifera) as antioxidant, anti-acetylcholinesterase and inhibition of lipopolysaccharide-induced nitric oxide production in murine macrophages.
- Source :
-
International journal of food sciences and nutrition [Int J Food Sci Nutr] 2012 Mar; Vol. 63 (2), pp. 187-93. Date of Electronic Publication: 2011 Sep 13. - Publication Year :
- 2012
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Abstract
- The multifunctional bioactive materials were prepared from Enteromorpha prolifera by enzyme-assisted extraction using four proteases and seven carbohydrases, and the biological activities of the enzyme-assisted extracts were evaluated as antioxidant, anti-acetylcholinesterase (AChE) and anti-inflammatory effect as the measures of inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW264.7 cells. The enzyme-assisted extracts were rich in polyphenols in the range 124 ± 4.2 to 844 ± 9.1 mg/100 g and flavonoids in the range 453 ± 6.0 to 675 ± 5.2 mg/100 g, and Protamex and Viscozyme extracts, which were rich in polyphenols and flavonoids, showed the highest 2,2-diphenyl-1-picrylhydrazyl scavenging, hydrogen peroxide scavenging, ferrous ion chelating and reducing power. Flavourzyme extract (89.92%) and Promozyme extract (93.64%) showed the highest AChE inhibitory activities at the concentration of 1.0 mg/ml. All enzyme-assisted extracts showed no cytotoxic effect on RAW264.7 cells at the tested concentration and significantly inhibited the LPS-induced NO production in RAW264.7 cells.
- Subjects :
- Acetylcholinesterase metabolism
Animals
Anti-Inflammatory Agents isolation & purification
Antioxidants analysis
Biphenyl Compounds metabolism
Cell Line
Cholinesterase Inhibitors isolation & purification
Ferrous Compounds metabolism
Hydrogen Peroxide metabolism
Lipopolysaccharides
Macrophages drug effects
Macrophages metabolism
Mice
Peptide Hydrolases metabolism
Picrates metabolism
Plant Preparations chemistry
Plants, Edible chemistry
Polyphenols isolation & purification
Polyphenols pharmacology
Anti-Inflammatory Agents pharmacology
Antioxidants pharmacology
Cholinesterase Inhibitors pharmacology
Nitric Oxide biosynthesis
Plant Preparations pharmacology
Seaweed chemistry
Ulva chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1465-3478
- Volume :
- 63
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of food sciences and nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 21913802
- Full Text :
- https://doi.org/10.3109/09637486.2011.616486