Back to Search Start Over

Identification and characterization of apelin peptides in bovine colostrum and milk by liquid chromatography-mass spectrometry.

Authors :
Mesmin C
Fenaille F
Becher F
Tabet JC
Ezan E
Source :
Journal of proteome research [J Proteome Res] 2011 Nov 04; Vol. 10 (11), pp. 5222-31. Date of Electronic Publication: 2011 Oct 13.
Publication Year :
2011

Abstract

Apelin peptides were recently identified as endogenous ligands of the APJ receptor. It has been hypothesized that these peptides are initially provided to the newborn by nursing and might be involved in gastrointestinal tract development. As apelin peptides may have different effects on the APJ receptor as a function of their size, knowledge of their exact structure in early milk is essential to clarify their action in gastrointestinal tract development. Bovine colostrum is thought to contain high concentrations of a wide diversity of apelin peptides, but none of them have yet been rigorously characterized. To identify and monitor apelin peptides in bovine colostrum, we developed a cation exchange extraction step followed by untargeted liquid chromatography coupled to high resolution and high mass accuracy mass spectrometry (LTQ-Orbitrap). Using this approach, we characterized 46 endogenous apelin peptides in bovine colostrum, which varied in relative abundance from one colostrum to another. Mature as well as commercial milk samples were also studied. Taken together, our data demonstrate that the multiplicity and variability of apelin peptides are biologically relevant and change during milk maturation to reach a more constant composition in mature milk.

Details

Language :
English
ISSN :
1535-3907
Volume :
10
Issue :
11
Database :
MEDLINE
Journal :
Journal of proteome research
Publication Type :
Academic Journal
Accession number :
21939284
Full Text :
https://doi.org/10.1021/pr200725x