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Virulence of an exotoxin A-deficient strain of Pseudomonas aeruginosa toward the silkworm, Bombyx mori.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2011 Dec; Vol. 51 (6), pp. 407-14. Date of Electronic Publication: 2011 Sep 17. - Publication Year :
- 2011
-
Abstract
- We studied the contribution of exotoxin A to the virulence of Pseudomonas aeruginosa against the silkworm, Bombyx mori. First, an exotoxin A-deficient mutant strain (PAO1toxA) was created, and its virulence compared with that of the parental PAO1 strain. In a short-term mortality assay, the mutant harboring pBBR1MCS2 did not kill B. mori until 120 h after inoculation and complementation of the corresponding gene in trans restored the strain's virulence. Next, to ascertain whether or not it lost all virulence, PAO1toxA (pBBR1MCS2, pGFP) was used in a long-term mortality assay. B. mori inoculated with the mutant strain did not die until early in the 5th instar (240 h after inoculation). However, 50% of the inoculated B. mori died late in the 5th instar or in the early pupal stage (408 h after inoculation). All had died by the pupal stage (600 h after inoculation). The mutant strain was isolated from dead larvae and cocoons. The bacterial population of PAO1toxA in hemolymph reached 4.77 × 10(7) cfu/ml. These results indicated that exotoxin A acts as a virulence factor in B. mori and that other virulence factor(s) are involved during the late stages of infection.<br /> (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Bacterial Load
Bombyx physiology
Gene Deletion
Genetic Complementation Test
Hemolymph microbiology
Larva microbiology
Larva physiology
Pseudomonas aeruginosa growth & development
Survival Analysis
Virulence
Pseudomonas aeruginosa Exotoxin A
ADP Ribose Transferases deficiency
ADP Ribose Transferases metabolism
Bacterial Toxins metabolism
Bombyx microbiology
Exotoxins deficiency
Exotoxins metabolism
Pseudomonas aeruginosa pathogenicity
Virulence Factors deficiency
Virulence Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 51
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 21945328
- Full Text :
- https://doi.org/10.1016/j.micpath.2011.09.002