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[SUMO-2/3 can covalently bind to progesterone receptor B to regulate its transcriptional activity].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2011 Sep; Vol. 31 (9), pp. 1493-7. - Publication Year :
- 2011
-
Abstract
- Objective: To investigate whether progesterone receptor B (PRB) can be sumoylated by SUMO-2/3 and the effect of sumoylation on PRB transcriptional activity.<br />Methods: SUMO-2/3 cDNA was amplified from MCF-7 cDNA and cloned into the eukaryotic expression vector pcDNA3-FLAG. The plasmid pXJ40-myc-PRB was cotransfected with pcDNA3FLAG-SUMO2, pcDNA3FLAG-SUMO3 or the mock control into 293T cells, and PRB sumoylation was detected by immunoprecipitation and Western blotting. The effect of PRB sumoylation on its transcriptional activity was determined using reporter luciferase assay.<br />Results: pcDNA3FLAG-SUMO2 and pcDNA3FLAG-SUMO3 vectors were successfully constructed. SUMO-2/3 could bind covalently to PRB and increase its transcriptional dependent on the presence of progesterone.<br />Conclusion: PRB can be sumoylated by SUMO-2/3 and its function is regulated by this modification.
- Subjects :
- Animals
Cell Line
Humans
Plasmids genetics
Receptors, Progesterone metabolism
Small Ubiquitin-Related Modifier Proteins metabolism
Transcription, Genetic
Transfection
Ubiquitination
Ubiquitins metabolism
Receptors, Progesterone genetics
Small Ubiquitin-Related Modifier Proteins genetics
Ubiquitins genetics
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 31
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 21945751