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[Expressions of RANK, RANKL, and osteoprotegerin in male rats at different ages].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2011 Sep; Vol. 31 (9), pp. 1539-42. - Publication Year :
- 2011
-
Abstract
- Objective: To investigate the expression of receptor activator of nuclear factor-κB (RANK), its ligand RANKL, and osteoprotegerin, and observe the effects of αD3 on their expressions in male rats at different ages.<br />Methods: Wistar rats at 6 weeks, 6 months, and 24 months (n=15) were examined for mRNA expressions of RANK/RANKL and osteoprotegerin in the left proximal femur using RT-PCR and for their protein expressions in the right femur using immunohistochemistry. RANK/RANKL and osteoprotegerin expressions were also detected in another 15 rats aged 24 months following intragastric administration of 0.05 µg/kg αD3 (3 times a week for 10 weeks).<br />Results: Compared with 6-week-old rats, 6-month- and 24-month-old rats showed a 6.2-fold and 7.3-fold increase of RANKL mRNA expression, respectively (P<0.05), and osteoprotegerin mRNA levels increased slightly with age. αD3 treatment resulted in significantly increased expression of RANK in 24-month-old rats with a lowered RANKL/osteoprotegerin ratio. RANKL and osteoprotegerin were co-localized in the osteoblasts and chondrocytes. αD3 treatment also caused an increased expression of osteoprotegerin mRNA in 24-month-old rats.<br />Conclusion: The age-related increase of the ratio of RANKL/osteoprotegerin mRNA promotes osteoclast activity and bone turnover. αD3 has favorable effect on osteogenesis and suppress bone absorption in the femur possibly by reducing RANK expression and lowering RANKL/osteoprotegerin ratio.
- Subjects :
- Age Factors
Animals
Femur metabolism
Male
Osteoprotegerin genetics
RANK Ligand genetics
RNA, Messenger genetics
Rats
Rats, Wistar
Receptor Activator of Nuclear Factor-kappa B genetics
Chondrocytes metabolism
Osteoblasts metabolism
Osteoprotegerin metabolism
RANK Ligand metabolism
Receptor Activator of Nuclear Factor-kappa B metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 31
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 21945761