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Photosensitizing carrier proteins for photoinducible RNA interference.
- Source :
-
Bioconjugate chemistry [Bioconjug Chem] 2011 Nov 16; Vol. 22 (11), pp. 2222-6. Date of Electronic Publication: 2011 Oct 27. - Publication Year :
- 2011
-
Abstract
- RNA interference (RNAi) is being widely explored as a tool in functional genomics and tissue engineering, and in the therapy of intractable diseases, including cancer and neurodegenerative diseases. Recently, we developed a photoinducible RNAi method using photosensitizing carrier proteins, named CLIP-RNAi (CPP-linked RBP-mediated RNA internalization and photoinduced RNAi). Novel carrier proteins were designed for this study to establish a highly efficient delivery system for small interfering RNA (siRNA) or short hairpin RNA (shRNA) and to demonstrate light-dependent gene silencing. In addition, the results suggested that the dissociation of the siRNA (or shRNA) from carrier proteins in the cytoplasm is a critical event in CLIP-RNAi-mediated gene silencing.
- Subjects :
- Animals
CHO Cells
Carrier Proteins chemistry
Carrier Proteins genetics
Cricetinae
Cricetulus
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Humans
Plasmids genetics
Plasmids metabolism
RNA, Small Interfering chemistry
RNA, Small Interfering genetics
Carrier Proteins metabolism
RNA Interference
RNA, Small Interfering metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1520-4812
- Volume :
- 22
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Bioconjugate chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 21985108
- Full Text :
- https://doi.org/10.1021/bc200095a